Download PDF by Margaret Kielian, Thomas Mettenleiter, Marilyn Roossinck: Advances in Virus Research

By Margaret Kielian, Thomas Mettenleiter, Marilyn Roossinck

ISBN-10: 0128048212

ISBN-13: 9780128048214

ISBN-10: 0128052368

ISBN-13: 9780128052365

Published considering that 1953, Advances in Virus Research covers a various variety of in-depth studies, supplying a necessary review of the present box of virology.

  • Contain contributions from major authorities
  • Informs and updates on the entire newest advancements within the box of virology

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Extra resources for Advances in Virus Research

Sample text

2G). To compare screening strategies, as well as perform an orthologous investigation of HRV14’s human cell requirements, we next carried out a CRISPR/Cas9 screen using the exact same cell line and virus. We report this CRISPR/Cas9 HRV14 screen here for the first time. We stably expressed a human codon-optimized cDNA of S. pyogenes Cas9 in a population of HeLa-H1 cells (Fig. , 2014). After selection with hygromycin, the cell population was tested for Cas9 expression by immunoblotting as well as the ability to satisfactorily extinguish the expression Figure 2—Cont'd expression data (Affy net expression) is also given based on a microarray analysis of mRNA from the H1-HeLa cells used in the screen.

Park, A. , et al. (2009). Haploid genetic screens in human cells identify host factors used by pathogens. Science, 326, 1231–1235. Carette, J. , Blomen, V. , Camargo, F. , et al. (2010). Generation of iPSCs from cultured human malignant cells. Blood, 115, 4039–4042. Carette, J. , Wong, A. , Herbert, A. , et al. (2011). Ebola virus entry requires the cholesterol transporter Niemann-Pick C1. Nature, 477, 340–343. Cherry, S. (2011). RNAi screening for host factors involved in viral infection using Drosophila cells.

PLoS Neglected Tropical Diseases, 6, e1820. Lee, A. , & Whelan, S. P. (2014). A genome-wide small interfering RNA screen identifies host factors required for vesicular stomatitis virus infection. Journal of Virology, 88, 8355–8360. , Brass, A. , Xavier, R. , Liang, T. , et al. (2009). A genome-wide genetic screen for host factors required for hepatitis C virus propagation. Proceedings of the National Academy of Sciences of the United States of America, 106, 16410–16415. , Oliveira, N. , Cheney, K.

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Advances in Virus Research by Margaret Kielian, Thomas Mettenleiter, Marilyn Roossinck

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