Download e-book for kindle: Advanced Photon Counting: Applications, Methods, by Peter Kapusta, Michael Wahl, Rainer Erdmann

By Peter Kapusta, Michael Wahl, Rainer Erdmann

ISBN-10: 3319156357

ISBN-13: 9783319156354

ISBN-10: 3319156365

ISBN-13: 9783319156361

This quantity makes a speciality of Time-Correlated unmarried Photon Counting (TCSPC), a robust device permitting luminescence lifetime measurements to be made with excessive temporal solution, even on unmarried molecules. Combining spectrum and lifelong offers a “fingerprint” for picking out such molecules within the presence of a heritage. Used including confocal detection, this enables single-molecule spectroscopy and microscopy as well as ensemble measurements, establishing up an incredible variety of scorching existence technological know-how purposes resembling fluorescence lifetime imaging (FLIM) and dimension of Förster Resonant power move (FRET) for the research of protein folding and interplay. a number of technology-related chapters current either the fundamentals and present cutting-edge, particularly of TCSPC electronics, photon detectors and lasers. the rest chapters conceal a vast diversity of purposes and methodologies for experiments and knowledge research, together with the lifestyles sciences, illness facilities in diamonds, super-resolution microscopy, and optical tomography. The chapters detailing new techniques coming up from the mix of vintage TCSPC and fluorescence lifetime with tools in response to depth fluctuation characterize a very specified highlight.

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In: Periasamy A, So PTC (eds) Multiphoton microscopy in the biomedical sciences IX; Proceedings of SPIE 7183:71830D:1–71830D:12 19. Felekyan S, Kalinin S, Sanabria H, Valeri A, Seidel CAM (2012) Filtered FCS: species autoand cross-correlation functions highlight binding and dynamics in biomolecules. Chem Phys Chem 13:1036–1053 20. Eggeling C, Berger S, Brand L, Fries JR, Schaffer J, Volkmer A, Seidel CAM (2001) Data registration and selective single-molecule analysis using multi-parameter fluorescence detection.

3 Dark Counts and Afterpulsing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 Timing Resolution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Geometrical Factors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

SPADs for the visible range are based on silicon. There are two main types of Si-SPADs architecture available today: thick [13–15] and thin-junction devices [16, 17]. The main difference between these two designs is the thickness of the depletion region in which photon absorption takes place. Thick-junction SPADs usually feature a depletion region of a few tens of μm, whereas thin-junction SPADs only have a few μm thickness. 2 Detection Efficiency D [%] SPADs based on silicon can generally be used in the spectral range between 400 and 1,100 nm.

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Advanced Photon Counting: Applications, Methods, Instrumentation by Peter Kapusta, Michael Wahl, Rainer Erdmann


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